Article
Assembly of imported subunit 8 into the ATP synthase complex of isolated yeast mitochondria.
European journal of biochemistry - 10 Mar 1990
Law R H, Devenish R J, Nagley P
Abstract excerpt
This study concerns the assembly into a multisubunit enzyme complex of a small hydrophobic protein imported into isolated mitochondria. Subunit 8 of yeast mitochondrial ATPase (normally a mitochondrial gene product) was expressed in vitro as a chimaeric precursor N9L/Y8-1, which includes an N-terminal-cleavable transit peptide to direct its import into mitochondria. Assembly into the enzyme complex of the...
Topics
- Adenosine Triphosphatases
- Antibodies, Monoclonal
- Cell Fractionation
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Vectors
- Immunosorbent Techniques
- Mitochondria
- Mutation
