Mutational analysis of ligand binding activity of beta 2 adrenergic receptor expressed in Escherichia coli.

doi:10.1002/j.1460-2075.1990.tb07453.x

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Article

Mutational analysis of ligand binding activity of beta 2 adrenergic receptor expressed in Escherichia coli.

The EMBO journal - 1 Sept 1990

Breyer R M, Strosberg A D, Guillet J G

Abstract excerpt

A novel in situ screening procedure was used to identify neutral mutations in the human beta 2 adrenergic receptor (beta 2AR). The coding region of the human beta 2AR gene was subcloned under transcriptional control of an inducible T7 promoter and used to transform Escherichia coli. Colonies expr...

Topics

Amino Acid Sequence
Escherichia coli
Humans
Iodocyanopindolol
Kinetics
Ligands
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Pindolol
Plasmids
Receptors, Adrenergic, beta
Recombinant Proteins
Restriction Mapping

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