Article
Ouabain-resistant mutants of the rat Na,K-ATPase alpha 2 isoform identified by using an episomal expression vector.
Molecular and cellular biology - 1 Apr 1990
Canfield V, Emanuel J R, Spickofsky N, Levenson R, Margolskee R F
Abstract excerpt
Site-directed mutagenesis was used to identify residues responsible for the greater than 1,000-fold difference in ouabain sensitivity between the rat Na,K-ATPase alpha 1 and alpha 2 isoforms. A series of mutagenized cDNAs was constructed that replaced residues of the rat alpha 2 subunit with the corresponding residues from the rat alpha 1 subunit. These cDNAs were cloned into a mammalian episomal expression...
Topics
- Amino Acid Sequence
- Animals
- Cell Division
- Cell Line
- Cloning, Molecular
- Genetic Vectors
- Humans
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Ouabain