A single amino acid change in CUP2 alters its mode of DNA binding.

doi:10.1128/mcb.10.9.4778-4787.1990

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Article

A single amino acid change in CUP2 alters its mode of DNA binding.

Molecular and cellular biology - 1 Sept 1990

Buchman C, Skroch P, Dixon W, Tullius T D, Karin M

Abstract excerpt

CUP2 is a copper-dependent transcriptional activator of the yeast CUP1 metallothionein gene. In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc). Using mobility shift, methylation interference, and DNase I and hydroxyl radical footprinting assays, we examined the interaction of...

Topics

Base Composition
Base Sequence
Cloning, Molecular
Copper
DNA, Fungal
DNA-Binding Proteins
Deoxyribonuclease I
Free Radicals
Fungal Proteins
Gene Expression Regulation, Fungal
Genes, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hydroxides

Hydroxyl Radical

Metallothionein

Models, Molecular

Molecular Sequence Data

Mutation

Nucleic Acid Conformation

Source

Open in PubMed Open full text

Identifiers

PMID: 2167439
DOI: 10.1128/mcb.10.9.4778-4787.1990
PMCID: PMC361081

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2167439-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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