Chromatin immunoprecipitation assay for analyzing histone H3-mediated regulation of copper homeostasis.

doi:10.1016/bs.mie.2026.05.030

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Article

Chromatin immunoprecipitation assay for analyzing histone H3-mediated regulation of copper homeostasis.

Methods in enzymology - 1 Jan 2026

Singh Sakshi, Sahu Rakesh Kumar, Tomar Raghuvir Singh

Abstract excerpt

Histone tails undergo various post-translational modifications that aid in recruiting effector proteins involved in diverse transcriptional responses. Our previous work explored the role of histone H3 N-terminal residues and their modifications on the transcriptional regulation of the CUP1 metallothionein gene during cellular copper stress. To pinpoint critical histone residues, we used complementary methods,...

Topics

Copper
Histones
Homeostasis
Chromatin Immunoprecipitation
Metallothionein
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae
Promoter Regions, Genetic
Gene Expression Regulation, Fungal
Mutation

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Open in PubMed

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PMID: 42624568
DOI: 10.1016/bs.mie.2026.05.030

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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