Article
Genetics of mouse hepatitis virus transcription: identification of cistrons which may function in positive and negative strand RNA synthesis.
Virology - 1 Aug 1990
Schaad M C, Stohlman S A, Egbert J, Lum K, Fu K, Wei T, Baric R S
Abstract excerpt
A panel of 26 temperature-sensitive mutants of MHV-A59 were selected by mutagenesis with either 5-fluorouracil or 5-azacytidine. Complementation analysis revealed the presence of one RNA+ and five RNA- complementation groups. None of the RNA- complementation groups transcribed detectable levels of positive- or negative-stranded RNA at the restrictive temperature. Temperature shift experiments after the onset of...
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