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Article

Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.

Nucleic acids research - 11 Dec 1994

Sekiguchi J, Shuman S

Abstract excerpt

Vaccinia DNA topoisomerase binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'(C/T)CCTT decreases in the scissile strand. Distinctive aspects of noncovalent versus covalent interaction emerge from analysis of the binding properties of Topo(Phe-274), a mutated protein which is unable to cleave DNA, but which binds DNA noncovalently. Whereas DNA cleavage by wild type...

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Identifiers

PMID: 7816626
DOI: 10.1093/nar/22.24.5360
PMCID: PMC332083

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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