Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.

doi:10.1093/nar/22.24.5360

Back to search

Article

Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA.

Nucleic acids research - 11 Dec 1994

Sekiguchi J, Shuman S

Abstract excerpt

Vaccinia DNA topoisomerase binds duplex DNA and forms a covalent adduct at sites containing a conserved sequence element 5'(C/T)CCTT decreases in the scissile strand. Distinctive aspects of noncovalent versus covalent interaction emerge from analysis of the binding properties of Topo(Phe-274), a...

Topics

Base Sequence
Binding Sites
Conserved Sequence
DNA
DNA Topoisomerases, Type I
DNA-Binding Proteins
Molecular Sequence Data
Mutation
Substrate Specificity
Vaccinia virus

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.