Article
Loss of DNA-binding and new transcriptional trans-activation function in polyomavirus large T-antigen with mutation of zinc finger motif.
Nucleic acids research - 11 May 1990
Bergqvist A, Nilsson M, Bondeson K, Magnusson G
Abstract excerpt
A putative zinc finger in polyomavirus large T-antigen was investigated. We were unable to demonstrate unequivocally a requirement for zinc in specific DNA-binding using the chelating agent 1, 10-phenanthroline. An involvement of the putative zinc finger in specific DNA-binding was nevertheless s...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Polyomavirus Transforming
- Cell Line
- DNA Replication
- DNA, Viral
- DNA-Binding Proteins
- HeLa Cells
- Humans
- Metalloproteins
- Molecular Sequence Data
- Mutation
- Phenanthrolines
- Polyomavirus
- Promoter Regions, Genetic
- Transcription, Genetic
- Transcriptional Activation
- Zinc