The DNA gyrase of Escherichia coli participates in the formation of a spontaneous deletion by recA-independent recombination in vivo.

doi:10.1007/BF00391737

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Article

The DNA gyrase of Escherichia coli participates in the formation of a spontaneous deletion by recA-independent recombination in vivo.

Molecular & general genetics : MGG - 1 Feb 1990

Miura-Masuda A, Ikeda H

Abstract excerpt

A system for detecting a spontaneous deletion in Escherichia coli was developed and the role of DNA gyrase in deletion formation was studied. A derivative of lambda plac5, lambda AM36, was isolated in which whole pBR322 DNA was inserted in the lacZ gene and 227 bp of the lac gene duplicated at bo...

Topics

Chromosome Deletion
Cloning, Molecular
DNA Topoisomerases, Type II
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Lac Operon
Mutation
Oxolinic Acid
Plasmids
Rec A Recombinases
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
Temperature

Source

Open in PubMed

Identifiers

PMID: 2160049
DOI: 10.1007/BF00391737

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2160049-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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