Article
Phenotypic difference between Δ(srl-recA)306 and ΔrecA::Km elucidated by next-generation sequencing combined with a long-PCR system.
The Journal of general and applied microbiology - 17 Mar 2017
Suzuki Shingo, Kaidow Akihiro, Meya Takuma, Masuya Anri, Shiina Takashi
Abstract excerpt
Many significant gene mutations in E. coli have contributed to the development of genetics. Among these, a commonly used recA mutation, Δ(srl-recA)306 has been sequenced by a next-generation sequencer combined with a long PCR. An original report described that Δ(srl-recA)306 cells were deleted from srlR to recA genes in their genome. The next-generation sequencer enables more accurate details to be determined. We...
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