Article
A specific terminal structure is required for Ty1 transposition.
Genes & development - 1 Mar 1990
Eichinger D J, Boeke J D
Abstract excerpt
Yeast retrotransposon Ty1 directs the synthesis of virus-like particles (VLPs) consisting of Ty1-encoded proteins, RNA, and reverse transcripts. Ty1 reverse transcripts, tagged with a selectable marker and found within VLPs, are capable of transposing into naked target DNA in vitro. Cassettes consisting of a Ty long terminal repeat (LTR), or delta, marked with supF, and flanked by appropriate restriction sites...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Fungal
- Molecular Sequence Data
- Mutation
- Repetitive Sequences, Nucleic Acid
- Saccharomyces cerevisiae
- Transcription, Genetic
