Article
Distamycin inhibition of topoisomerase I-DNA interaction: a mechanistic analysis.
Nucleic acids research - 25 Apr 1990
Mortensen U H, Stevnsner T, Krogh S, Olesen K, Westergaard O, Bonven B J
Abstract excerpt
Inhibition of eukaryotic DNA topoisomerase I by the minor groove binding ligand, distamycin A, was investigated. Low concentrations of the ligand selectively prevented catalytic action at a high affinity topoisomerase I binding sequence. A restriction enzyme protection assay indicated that the catalytic cycle was blocked at the binding step. Distamycin binding sites on DNA were localized by hydroxyl radical...
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