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Article

Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors.

The EMBO journal - 1 May 1993

Freudenreich C H, Kreuzer K N

Abstract excerpt

We have analyzed the DNA sequence requirements for cleavage of a 30 bp oligonucleotide that contains a strong bacteriophage T4 type II topoisomerase site. A novel method was used to generate substrates with each of the four nucleotides at 10 positions surrounding the cleavage site, and mutant substrates were also prepared for the four internal positions of the staggered cleavage site. The substrates were tested...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 8387918
DOI: 10.1002/j.1460-2075.1993.tb05857.x
PMCID: PMC413430

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Source receipt: pubmed, retrieved 7 Sept 2026.

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