Article
Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors.
The EMBO journal - 1 May 1993
Freudenreich C H, Kreuzer K N
Abstract excerpt
We have analyzed the DNA sequence requirements for cleavage of a 30 bp oligonucleotide that contains a strong bacteriophage T4 type II topoisomerase site. A novel method was used to generate substrates with each of the four nucleotides at 10 positions surrounding the cleavage site, and mutant sub...
Topics
- Bacteriophage T4
- Base Sequence
- Binding Sites
- DNA Mutational Analysis
- DNA Topoisomerases, Type II
- DNA, Viral
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Substrate Specificity
- Topoisomerase II Inhibitors