Article
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Effect of pyrophosphorolysis and metal ions.
The Journal of biological chemistry - 15 May 1990
Tabor S, Richardson C C
Abstract excerpt
Pyrophosphorolysis by bacteriophage T7 DNA polymerase leads to the degradation of specific dideoxynucleotide-terminated fragments on DNA sequencing gels. This reaction can be prevented by pyrophosphatase. It is also inhibited by a high concentration of dNTPs; only the dNTP complementary to the ne...
Topics
- Animals
- Autoanalysis
- Base Sequence
- DNA
- DNA-Directed DNA Polymerase
- Deoxyadenine Nucleotides
- Deoxycytosine Nucleotides
- Deoxyguanine Nucleotides
- Deoxyribonucleotides
- Dideoxynucleotides
- Drosophila
- Genetic Carrier Screening
- Magnesium
- Manganese
- Molecular Sequence Data
- Mutation
- Phosphates
- Polymerase Chain Reaction