Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutation detection by solid phase primer extension.

Human mutation - 1 Jan 1996

Shumaker J M, Metspalu A, Caskey C T

Abstract excerpt

A mutation analysis method based upon a wild-type DNA sequence is presented. Oligonucleotides were utilized for primer extension by T7 DNA polymerase to discriminate between wild-type and mutant sequences in two solid phase approaches. 1. Oligonucleotides were annealed to an immobilized template, extended with fluorescent dideoxynucleotides (ddNTPs), and analyzed on an automated fluorescent DNA sequencer. The...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mutation
Share to Base Sequence
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hypoxanthine Phosphoribosyltransferase

Share to Molecular Sequence Data

Share to Nucleic Acid Hybridization

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8723685
DOI: 10.1002/(SICI)1098-1004(1996)7:4<346::AID-HUMU9>3.0.CO;2-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutation detection by solid phase primer extension.PMID 8723685Read ↗

Sensitive and fast mutation detection by solid phase chemical cleavage.Semantic proximity Read ↗

Competitive oligonucleotide single-base extension combined with mass spectrometric detection for mutation screening.Semantic proximity Read ↗

Detection of mutant sequences by single-strand conformation polymorphism analysis.Semantic proximity Read ↗

Solid phase in vitro mutagenesis using plasmid DNA template.Semantic proximity Read ↗

Combination of His-tagged T4 endonuclease VII with microplate array diagonal gel electrophoresis for high-throughput mutation scanning.Semantic proximity Read ↗

Detection of point mutations using PNA-containing electrophoresis matrices.Semantic proximity Read ↗

Mutant DNA polymerase for improved detection of single-nucleotide variations in microarrayed primer extension.Semantic proximity Read ↗

Enzymatic mutation detection. Procedure for screening and mapping of mutations by immobilised endonuclease VII.Semantic proximity Read ↗

A new technology for mutation detection.Semantic proximity Read ↗

Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence.Semantic proximity Read ↗

Mutant oligonucleotide extension amplification: a nonlabeling polymerase-chain-reaction-based assay for the detection of point mutations.Semantic proximity Read ↗

The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.