Article
Transcription in vivo directed by consensus sequences of E.coli promoters: their context heavily affects efficiencies and start sites.
Nucleic acids research - 11 Mar 1990
Jacquet M A, Reiss C
Abstract excerpt
We studied in vivo transcription and gene expression directed by a series of synthetic sequences, bearing the consensus hexamer (CH) pair of E.coli promoters in various contexts. The results demonstrate that, for the contexts tested, the CH pair supports transcription activity and gene expression, whether the spacer linking them is AT or GC rich, or is as short as 14 bp or as large as 26 bp (standard size 17 bp)....
Topics
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- Chloramphenicol Resistance
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Phenotype
- Plasmids