Article
Intracellular localization of Na,K-ATPase alpha2 subunit mutants.
Archives of biochemistry and biophysics - 15 Oct 1997
Coppi M V, Guidotti G
Abstract excerpt
The Na,K-ATPase is an essential plasma membrane transporter of mammalian cells composed of two subunits, alpha and beta, of which there are several isoforms. We investigated the effect of a substitution, S364P, on the subcellular localization and enzymatic activity of the wild-type alpha2 and alp...
Topics
- Animals
- Binding Sites
- Biological Transport
- Blotting, Western
- COS Cells
- Cell Fractionation
- Endoplasmic Reticulum
- Genes, Reporter
- HeLa Cells
- Humans
- Mutagenesis, Site-Directed
- Mutation
- Orthomyxoviridae
- Ouabain
- Phosphorylation
- Protein Binding
- Recombinant Fusion Proteins
- Sodium-Potassium-Exchanging ATPase
