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Article

Mutation of a consensus purine nucleotide binding site in the adeno-associated virus rep gene generates a dominant negative phenotype for DNA replication.

Journal of virology - 1 Apr 1990

Chejanovsky N, Carter B J

Abstract excerpt

Adeno-associated virus (AAV) contains a multifunctional nonstructural gene, rep, which is required for AAV DNA replication and has pleiotropic effects on positive and negative regulation of gene expression. All of the parvovirus nonstructural genes contain a region of highly conserved amino acid...

Topics

Amino Acid Sequence
Base Sequence
Binding Sites
Cell Line
DNA Replication
DNA, Viral
Dependovirus
Genes, Viral
Humans
Molecular Sequence Data
Mutation
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Purine Nucleotides

Viral Proteins

Virus Replication

Source

Open in PubMed Open full text

Identifiers

PMID: 2157057
DOI: 10.1128/JVI.64.4.1764-1770.1990
PMCID: PMC249314

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2157057-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Mutation of a consensus purine nucleotide binding site in the adeno-associated virus rep gene generates a dominant negative phenotype for DNA replication.PMID 2157057Read ↗

Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.Semantic proximity Read ↗

Analysis of adeno-associated virus (AAV) wild-type and mutant Rep proteins for their abilities to negatively regulate AAV p5 and p19 mRNA levels.Semantic proximity Read ↗

Comprehensive mutagenesis maps the effect of all single-codon mutations in the AAV2 rep gene on AAV production.Semantic proximity Read ↗

Sequences required for coordinate induction of adeno-associated virus p19 and p40 promoters by Rep protein.Semantic proximity Read ↗

Identification of mutant adeno-associated virus Rep proteins which are dominant-negative for DNA helicase activity.Semantic proximity Read ↗

Characterization of adeno-associated virus rep protein inhibition of adenovirus E2a gene expression.Semantic proximity Read ↗

Cell lines inducibly expressing the adeno-associated virus (AAV) rep gene: requirements for productive replication of rep-negative AAV mutants.Semantic proximity Read ↗

Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep proteinSemantic proximity Read ↗

Human Adeno-Associated Virus Type 5 Is Only Distantly Related to Other Known Primate Helper-Dependent ParvovirusesSemantic proximity Read ↗

A Novel Gene Expression Control System and Its Use in Stable, High-Titer 293 Cell-Based Adeno-Associated Virus Packaging Cell LinesSemantic proximity Read ↗

Evidence for Packaging of rep-cap Sequences into Adeno-Associated Virus (AAV) Type 2 Capsids in the Absence of Inverted Terminal Repeats: a Model for Generation of rep -Positive AAV ParticlesSemantic proximity Read ↗

A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19Semantic proximity Read ↗

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