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Article

Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.

Journal of virology - 1 Jul 1992

McCarty D M, Ni T H, Muzyczka N

Abstract excerpt

The adeno-associated virus (AAV) Rep protein is required for both viral DNA replication and transactivation of the AAV promoters. Here we report the effects of mutations in the rep gene on transcription and replication in vivo and terminal repeat binding and terminal resolution site (trs) endonuclease activities in vitro. In all, we examined 10 in-frame deletions and 14 amino acid substitution mutations at eight...

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Share to DNA, Viral

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Complementation Test

Share to Molecular Sequence Data

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Share to Repetitive Sequences, Nucleic Acid

Share to Transcription, Genetic

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Identifiers

PMID: 1318396
DOI: 10.1128/JVI.66.7.4050-4057.1992
PMCID: PMC241207

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.PMID 1318396Read ↗

Mutation of a consensus purine nucleotide binding site in the adeno-associated virus rep gene generates a dominant negative phenotype for DNA replication.Semantic proximity Read ↗

Adeno-associated virus (AAV) site-specific recombination does not require a Rep-dependent origin of replication within the AAV terminal repeatSemantic proximity Read ↗

Characterization of adeno-associated virus rep protein inhibition of adenovirus E2a gene expression.Semantic proximity Read ↗

Comprehensive mutagenesis maps the effect of all single-codon mutations in the AAV2 rep gene on AAV production.Semantic proximity Read ↗

Identification of mutant adeno-associated virus Rep proteins which are dominant-negative for DNA helicase activity.Semantic proximity Read ↗

Evidence for Packaging of rep-cap Sequences into Adeno-Associated Virus (AAV) Type 2 Capsids in the Absence of Inverted Terminal Repeats: a Model for Generation of rep -Positive AAV ParticlesSemantic proximity Read ↗

Analysis of adeno-associated virus (AAV) wild-type and mutant Rep proteins for their abilities to negatively regulate AAV p5 and p19 mRNA levels.Semantic proximity Read ↗

Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep proteinSemantic proximity Read ↗

Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.Semantic proximity Read ↗

A Rep recognition sequence is necessary but not sufficient for nicking of DNA by adeno-associated virus type-2 Rep proteins.Semantic proximity Read ↗

Role of the terminal repeat GAGC trimer, the major Rep78 binding site, in adeno-associated virus DNA replication.Semantic proximity Read ↗

Adeno-associated virus type 2 p5 promoter: a rep-regulated DNA switch element functioning in transcription, replication, and site-specific integration.Semantic proximity Read ↗

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