Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.

doi:10.1016/0022-2836(90)90127-8

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Article

Design and characterization of Escherichia coli mutants devoid of Ap4N-hydrolase activity.

Journal of molecular biology - 20 Mar 1990

Lévĕque F, Blanchin-Roland S, Fayat G, Plateau P, Blanquet S

Abstract excerpt

Escherichia coli strains with abnormally high concentrations of bis(5'-nucleosidyl)-tetraphosphates (Ap4N) were constructed by disrupting the apaH gene that encodes Ap4N-hydrolase. Variation deletions and insertions were also introduced in apaG and ksgA, two other cistrons of the ksgA apaGH opero...

Topics

Acid Anhydride Hydrolases
Aminoglycosides
Anti-Bacterial Agents
Blotting, Southern
Cloning, Molecular
Drug Resistance, Microbial
Escherichia coli
Genes, Bacterial
Mutation
Operon
Phenotype
Phosphoric Diester Hydrolases
Plasmids
Restriction Mapping
Temperature

Source

Open in PubMed

Identifiers

PMID: 2157025
DOI: 10.1016/0022-2836(90)90127-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2157025-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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