Article
Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay.
Memorias do Instituto Oswaldo Cruz - 1 Mar 2011
Maschmann Raquel de A, Verza Mirela, Silva Marcia Sn, Sperhacke Rosa Dea, Ribeiro Marta O, Suffys Philip Noel, Gomes Harrison Magdinier, Tortoli Enrico, Marcelli Fiorella, Zaha Arnaldo, Rossetti Maria Lucia R
Abstract excerpt
We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were...
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