Article
In house reverse line hybridization assay for rapid detection of susceptibility to rifampicin in isolates of Mycobacterium tuberculosis.
Journal of microbiological methods - 1 Nov 2006
Senna Simone G, Gomes Harrison M, Ribeiro Marta O, Kristki Afrânio L, Rossetti Maria Lucia R, Suffys Philip N
Abstract excerpt
We developed a Reverse Hybridization Assay (RHA) slightly modified from the Rifoligotyping assay and analyzed the presence of mutations in a specific region of rpoB gene in 157 isolates (90 rifampin-resistant and 67 rifampin sensitive) of Mycobacterium tuberculosis from patients attended in South and Southeast region of Brazil. Comparing to standardized drug susceptibility testing results, the sensitivity and...
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