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Article

Expression and targeting of intracellular antibodies in mammalian cells.

The EMBO journal - 1 Jan 1990

Biocca S, Neuberger M S, Cattaneo A

Abstract excerpt

Genes encoding the heavy and light chains of a hapten-specific IgM antibody were modified by site-directed mutagenesis to destroy the hydrophobic leader sequences and allow expression in the cytoplasm of non-lymphoid cells. The in situ assembly of the mutant heavy and light chains was tested in transfected cell lines by immunofluorescence using anti-idiotypic antibodies. A positive diffuse cytoplasmic staining...

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Identifiers

PMID: 2153072
DOI: 10.1002/j.1460-2075.1990.tb08085.x
PMCID: PMC551635

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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