Article
Expression and targeting of intracellular antibodies in mammalian cells.
The EMBO journal - 1 Jan 1990
Biocca S, Neuberger M S, Cattaneo A
Abstract excerpt
Genes encoding the heavy and light chains of a hapten-specific IgM antibody were modified by site-directed mutagenesis to destroy the hydrophobic leader sequences and allow expression in the cytoplasm of non-lymphoid cells. The in situ assembly of the mutant heavy and light chains was tested in t...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Viral, Tumor
- Base Sequence
- Cell Nucleus
- Cytoplasm
- Fluorescent Antibody Technique
- Gene Expression
- Genes, Immunoglobulin
- Haptens
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains