Article
Paradoxical enhancement of interleukin-2-mediated cytotoxicity against K562 cells by addition of a low dose of methotrexate.
Cancer immunology, immunotherapy : CII - 1 Jan 1990
Nakarai T, Ueno Y, Ueno Y, Koizumi S
Abstract excerpt
In vitro effects of methotrexate (MTX) on interleukin-2(IL-2)-mediated cytotoxicity of peripheral blood mononuclear cells (PBMC) were studied. PBMC were incubated with human recombinant IL-2 (25 U/ml) for 72 h; during the last 24 h, various concentrations (10 pM-1 microM) of MTX were added to the...
Topics
- Adult
- Antigens, Differentiation
- Antineoplastic Combined Chemotherapy Protocols
- Cell Survival
- Dose-Response Relationship, Drug
- Drug Synergism
- Humans
- Immunity, Cellular
- Interleukin-2
- Killer Cells, Lymphokine-Activated
- Killer Cells, Natural
- Leukemia, Experimental
- Leukocytes, Mononuclear
- Methotrexate
- Phenotype
- Receptors, Fc
- Receptors, IgG
- Tumor Cells, Cultured