Article
A common telomeric gene silencing assay is affected by nucleotide metabolism.
Molecular cell - 8 Apr 2011
Rossmann Marlies P, Luo Weijun, Tsaponina Olga, Chabes Andrei, Stillman Bruce
Abstract excerpt
Telomere-associated position-effect variegation (TPEV) in budding yeast has been used as a model for understanding epigenetic inheritance and gene silencing. A widely used assay to identify mutants with improper TPEV employs the URA3 gene at the telomere of chromosome VII-L that can be counterselected with 5-fluoroorotic acid (5-FOA). 5-FOA resistance has been inferred to represent lack of transcription of URA3...
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