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Article

A phasmid optimised for protein design projects: pMAMPF.

Gene - 28 Sept 1990

Szardenings M, Collins J

Abstract excerpt

Protein design requires the rapid production of recombinant genes and active recombinant proteins, the latter in sufficient amounts for functional and physical studies. We present here the construction and application of a new phasmid vector system, using the fd phage origin, lambda pL promoter, ompA-leader sequence and pMB1 origin, which allows the preparation of secretable proteins in active form, mutagenesis...

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PMID: 2146188
DOI: 10.1016/0378-1119(90)90460-9

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