Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes.

Abstract excerpt

Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purified and used to probe yeast proteome microarrays. One-thousand-twenty-three interactions were...

Topics

• Cell Physiological Phenomena
• Immunoprecipitation
• Mitogen-Activated Protein Kinases
• Phenotype
• Protein Array Analysis