Article
[Spontaneous spectinomycin resistance mutations of the chloroplast rrn16 gene in Daucus carota callus lines].
Genetika - 1 Jan 2011
Filipenko E A, Sidorchuk Iu V, Deĭneko E V
Abstract excerpt
Bioballistic transformation of carrot Daucus carota L. callus cultures with a plasmid containing the aadA (aminoglycoside 3'-adenyltransferase) gene and subsequent selection oftransformants on a selective medium containing spectinomycin (100-500 mg/l) yielded ten callus lines resistant to this antibiotic. PCR analysis did not detect exogenous DNA in the genomes of spectinomycin-resistant calluses. Resistance...
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