Article

Transformation and regeneration of carrot (Daucus carota L.)

1998-01-01

Abstract excerpt

A protocol is presented for the efficient transformation of carrot (Daucus carota L. cv. Nantaise) by Agrobacterium tumefaciens. The binary vector contained the marker gene beta-glucuronidase (GUS), driven by the 35S promoter of cauliflower mosaic virus, and the nptII gene, which confers kanamycin resistance. Highest T-DNA transfer rates were obtained by co-cultivating bacteria with hypocotyl segments of dark-grow...

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Literature Corpus work: 59444089-9501-519c-a106-42b3f7b40e02
DOI: 10.1023/a:1009681725540

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