Article
Mutation proximal to the tRNA binding region of the Nicotiana plastid 16S rRNA confers resistance to spectinomycin.
Molecular & general genetics : MGG - 1 Aug 1991
Svab Z, Maliga P
Abstract excerpt
Nicotiana tabacum lines carrying maternally inherited resistance to spectinomycin were obtained by selection for green callus in cultures bleached by spectinomycin. Two levels of resistance was found. SPC1 and SPC2 seedlings are resistant to high levels (500 micrograms/ml), SPC23 seedlings are resistant to low levels (50 micrograms/ml) of spectinomycin. Lines SPC2 and SPC23 are derivatives of the SR1...
Topics
- Chloroplasts
- Cloning, Molecular
- DNA Mutational Analysis
- Drug Resistance
- Extrachromosomal Inheritance
- Mutation
- Plants, Toxic
- Plasmids
- RNA, Ribosomal, 16S
- Spectinomycin
- Nicotiana