Article
Spectinomycin resistance mutations in the rrn16 gene are new plastid markers in Medicago sativa.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Nov 2012
Dudas Brigitta, Jenes Barnabas, Kiss Gyorgy Botond, Maliga Pal
Abstract excerpt
We report here the isolation of spectinomycin-resistant mutants in cultured cells of Medicago sativa line RegenSY-T2. Spectinomycin induces bleaching of cultured alfalfa cells due to inhibition of protein synthesis on the prokaryotic type 70S plastid ribosomes. Spontaneous mutants resistant to spectinomycin bleaching were identified by their ability to form green shoots on plant regeneration medium containing...
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