Article
Chloroplast targeting of spectinomycin adenyltransferase provides a cell-autonomous marker for monitoring transposon excision in tomato and tobacco.
Molecular & general genetics : MGG - 25 Jul 1994
Scofield S R, Jones D A, Harrison K, Jones J D
Abstract excerpt
Antibiotic resistance genes can act as either cell autonomous or non-cell autonomous genetic markers with which to monitor the excision of plant transposons. To convert spectinomycin resistance from a non-cell autonomous resistance to cell autonomous resistance, a transit peptide for chloroplast localization from a petunia ribulose bisphosphate carboxylase (rbcS) gene was fused in-frame to the aadA gene, which...
Topics
- Base Sequence
- Chloroplasts
- Cloning, Molecular
- Codon
- DNA Transposable Elements
- Drug Resistance
- Genetic Markers
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nucleotidyltransferases