Article
[Studies on construction of artificial mutants of Cucumber mosaic virus satellite RNA and their biological activity].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 2005
Jin Bo, Chen Ji-Shuang, Zhang Hua-Rong
Abstract excerpt
Based on the full length cDNA clone of a Cucumber mosaic virus satellite RNA, which was 369nt in size, artificial mutants were developed by the method of error-prone PCR and DNA shuffling. The new satellite cDNAs were transcribed in vitro into ssRNA and pseudo-recombined with a helper Cucumber mosaic virus, which contains no satellite RNA. Sequence analysis showed that A to T/G or G to A replacement all the four...
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