Article
In vitro and in vivo bioactivity of recombinant human follicle-stimulating hormone and partially deglycosylated variants secreted by transfected eukaryotic cell lines.
Endocrinology - 1 Jul 1990
Galway A B, Hsueh A J, Keene J L, Yamoto M, Fauser B C, Boime I
Abstract excerpt
Recent studies have shown that Chinese hamster ovary (CHO) cells transfected with the FSH subunit genes secrete bioactive FSH. Here, we determined the in vitro and in vivo bioactivity of recombinant FSH produced by CHO mutant cells deficient in the glycosylation enzyme N-acetylglucosamine transferase-I (NAGT-), resulting in glycoproteins with asparagine-linked (GlcNAc)2(Mannose)5 oligosaccharides, or mutant cells...
Topics
- Animals
- Aromatase
- Biological Assay
- Carbohydrate Conformation
- Cell Line
- Cricetinae
- Female
- Follicle Stimulating Hormone
- Glucosyltransferases
- Glycosylation
- Granulosa Cells