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Article

[Improvement on PCR-CTPP: a SNP genotyping approach based on mismatch technique].

Yi chuan = Hereditas - 1 Feb 2011

Wang Ke, Zhang Jin-Tao, Yun Yu-Xia, Wu Xiao-Bing, Chen A-Qun, Wang Peng, Wang Kai-Juan, Zhang Jian-Ying, Dai Li-Ping

Abstract excerpt

To explore the technique principle of PCR with confronting two-pair primers (PCR-CTPP) and improve the accuracy of SNP genotyping by taking the 1298 locus of human gene MTHFR as an example, the reliability between conventional PCR-CTPP and improved PCR-CTPP was compared using reconstructed PCR-CTPP detecting system in terms of designing appropriate primers and optimizing annealing temperature and the final...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 21377976
DOI: 10.3724/sp.j.1005.2011.00182

Genes

MTHFR

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