Deoxyhypusine/hypusine formation on a 21,000-dalton cellular protein in a Neurospora crassa mutant in vivo and in vitro.

doi:10.1016/0304-4165(90)90003-f

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Article

Deoxyhypusine/hypusine formation on a 21,000-dalton cellular protein in a Neurospora crassa mutant in vivo and in vitro.

Biochimica et biophysica acta - 26 Feb 1990

Yang Y C, Chen K Y, Seyfzadeh M, Davis R H

Abstract excerpt

Hypusine formation on an 18,000-dalton cellular protein is a unique spermidine-dependent, post-translational modification that appears to be ubiquitous in mammalian cells. To determine whether this modification also exists in lower eukaryotes, we examined possible labeling in vitro and in vivo of...

Topics

Electrophoresis, Polyacrylamide Gel
Fungal Proteins
Isoelectric Focusing
Lysine
Mutation
Neurospora
Neurospora crassa
Polyamines

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