Article
Composition of complex I from Neurospora crassa and disruption of two "accessory" subunits.
Biochimica et biophysica acta - 1 Jan 2000
Marques Isabel, Duarte Margarida, Assunção Joana, Ushakova Alexandra V, Videira Arnaldo
Abstract excerpt
Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subunits, of which 35 are conserved in mammals. The 11.5 kDa and 14 kDa proteins, homologues of bovine IP15 and B16.6, respectively, are conserved among eukaryotes and belong to the membrane domain of the fungal enzyme. The corresponding genes were separately inactivated by repeat-induced point-mutations, and null-mutant...
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