Article
Expression and biochemical characterization of the human enzyme N-terminal asparagine amidohydrolase.
Biochemistry - 12 Apr 2011
Cantor Jason R, Stone Everett M, Georgiou George
Abstract excerpt
The enzymatic deamidation of N-terminal L-Asn by N-terminal asparagine amidohydrolase (NTAN1) is a feature of the ubiquitin-dependent N-end rule pathway of protein degradation, which relates the in vivo half-life of a protein to the identity of its N-terminal residue. Herein, we report the bacterial expression, purification, and biochemical characterization of human NTAN1 (hNTAN1). We show here that hNTAN1 is...
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