Article
Focusing mutations within random libraries to distinct areas: protein domain library generation by overlap extension.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Gratz Andreas, Jose Joachim
Abstract excerpt
Directed evolution is an often used approach toward new proteins with tailor-made properties. It consists of random variation of the coding sequence of a protein followed by an appropriate selection procedure or a suitable type of property read out. In many, if not all cases, it is of significant advantage to constrain the randomly mutagenized DNA sequence to that encoding a particular part of the protein or a...
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