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Article

Magnesium ion-dependent activation of the RecA protein involves the C terminus.

The Journal of biological chemistry - 2 May 2003

Lusetti Shelley L, Shaw Jeffrey J, Cox Michael M

Abstract excerpt

Optimal conditions for RecA protein-mediated DNA strand exchange include 6-8 mm Mg(2+) in excess of that required to form complexes with ATP. We provide evidence that the free magnesium ion is required to mediate a conformational change in the RecA protein C terminus that activates RecA-mediated DNA strand exchange. In particular, a "closed" (low Mg(2+)) conformation of a RecA nucleoprotein filament restricts DNA...

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Identifiers

PMID: 12595538
DOI: 10.1074/jbc.M212916200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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