Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 A) and biochemical basis.

Abstract excerpt

Change in specificity, caused by the mutations at P1 site, of the serine protease inhibitors of different families is reported in the literature, but Kunitz (STI) family inhibitors are almost unexplored in this regard. In this paper, we present the crystal structure of a P1 variant of winged bean chymotrypsin inhibitor (WCI) belonging to Kunitz (STI) family, supplemented by biochemical, phylogenetic and docking...

Topics

• Amino Acid Substitution
• Base Sequence
• Chymotrypsin
• Crystallography, X-Ray
• DNA Primers
• Genetic Variation
• Models, Molecular
• Mutagenesis, Site-Directed
• Polymorphism, Single Nucleotide
• Protease Inhibitors