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Article

Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes.

Journal of virology - 1 Mar 2011

Pu Szu-Yuan, Wu Ren-Huang, Yang Chi-Chen, Jao Tzu-Ming, Tsai Ming-Han, Wang Jing-Chyi, Lin Hui-Mei, Chao Yu-Sheng, Yueh Andrew

Abstract excerpt

Reverse genetics is a powerful tool to study single-stranded RNA viruses. Despite tremendous efforts having been made to improve the methodology for constructing flavivirus cDNAs, the cause of toxicity of flavivirus cDNAs in bacteria remains unknown. Here we performed mutational analysis studies to identify Escherichia coli promoter (ECP) sequences within nucleotides (nt) 1 to 3000 of the dengue virus type 2...

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Identifiers

PMID: 21228244
DOI: 10.1128/JVI.01986-10
PMCID: PMC3067970

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Source receipt: pubmed, retrieved 7 Sept 2026.

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