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Article

A simple method for developing an infectious cDNA clone of Japanese encephalitis virus.

Virus genes - 1 Feb 2017

Zheng Hao, Zheng Xuchen, Tong Wu, Liu Fei, Liang Chao, Wang Tao, Gao Fei, Li Liwei, Shan Tongling, Li Guoxin, Tong Guangzhi

Abstract excerpt

Flavivirus cDNA clones frequently demonstrate genetic instability in transformed bacteria, which hampers the construction and manipulation of cDNAs for infectious flaviviruses. In this study, we developed a stable, full-length cDNA clone, pJEHEN, of a GI JEV strain HEN0701 using a medium-copy-number pBR322 vector and propagating cDNA clones at room temperature. The virus vJEHEN recovered from the infectious clone...

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PMID: 27665292
DOI: 10.1007/s11262-016-1387-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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