Construction of a CMV promoter-driven Langat virus cDNA clone and reporter viruses

Abstract excerpt

Langat virus (LGTV) is a tick-borne member of the Flaviviridae family and a biosafety level 2 surrogate for studying tick-borne encephalitis virus (TBEV) replication and pathogenesis. Here, we report the construction of a plasmid encoding a cytomegalovirus (CMV) promoter-driven LGTV cDNA that initiates infection following direct transfection of mammalian cells. Incorporation of three introns eliminated viral cDNA...

Topics

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• CRISPR and Genetic Engineering
• Cytomegalovirus and herpesvirus research
• Herpesvirus Infections and Treatments
• Mosquito-borne diseases and control
• Viral gastroenteritis research and epidemiology
• Viral Infections and Immunology Research
• Viral Infections and Vectors
• Virus-based gene therapy research

Identifiers and source

Literature Corpus work

aa5d41c0-068d-5b53-bcda-af16dec04b13

DOI

10.1101/2025.11.13.688285