Article
Unstable side population phenotype of mouse spermatogonial stem cells in vitro.
The Journal of reproduction and development - 1 Apr 2011
Shinohara Takashi, Ishii Kei, Kanatsu-Shinohara Mito
Abstract excerpt
Stem cells of the side population (SP) phenotype are found in many self-renewing tissues and can be identified by their unique ability to effectively exclude the dye Hoechst 33342. We previously established a method for expanding spermatogonial stem cells (SSCs) in vitro, but the frequency of SSCs is only about 1 to 2%, limiting detailed SSC analyses. In this study, we sought to isolate SSCs from in vitro...
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