Article
Controlled translocation of individual DNA molecules through protein nanopores with engineered molecular brakes.
Nano letters - 9 Feb 2011
Rincon-Restrepo Marcela, Mikhailova Ellina, Bayley Hagan, Maglia Giovanni
Abstract excerpt
Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the α-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site...
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