Article
Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic beta2-microglobulin variant.
Proceedings of the National Academy of Sciences of the United States of America - 25 Jan 2011
Domanska Katarzyna, Vanderhaegen Saskia, Srinivasan Vasundara, Pardon Els, Dupeux Florine, Marquez Jose A, Giorgetti Sofia, Stoppini Monica, Wyns Lode, Bellotti Vittorio, Steyaert Jan
Abstract excerpt
Atomic-level structural investigation of the key conformational intermediates of amyloidogenesis remains a challenge. Here we demonstrate the utility of nanobodies to trap and characterize intermediates of β2-microglobulin (β2m) amyloidogenesis by X-ray crystallography. For this purpose, we selected five single domain antibodies that block the fibrillogenesis of a proteolytic amyloidogenic fragment of β2m...
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