Structural and aggregation behavior of the human γD-crystallin mutant E107A, associated with congenital nuclear cataract.

Article

Structural and aggregation behavior of the human γD-crystallin mutant E107A, associated with congenital nuclear cataract.

Molecular vision - 17 Dec 2010

Vendra Venkata Pulla Rao, Balasubramanian Dorairajan

Abstract excerpt

PURPOSE: To analyze the conformational features and aggregation properties of the mutant protein E107A human γD-crystallin (HGDC), associated with congenital nuclear cataract. METHODS: cDNAs of wild type and E107A mutant were cloned and expressed in BL21 (DE3) pLysS cells and the proteins isolate...

Topics

Cataract
Cell Line
Humans
Lens Nucleus, Crystalline
Microscopy, Confocal
Mutant Proteins
Mutation
Protein Structure, Quaternary
Transfection
gamma-Crystallins

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