Article
Investigating the structural and functional effects of mutating Asn glycosylation sites of horseradish peroxidase to Asp.
Applied biochemistry and biotechnology - 1 Jun 2011
Asad Sedigheh, Khajeh Khosro, Ghaemi Nasser
Abstract excerpt
Horseradish peroxidase (HRP) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors, and biocatalysis. Enhancement of HRP catalytic activity and/or stability would further increase its applications. One of the problems with heterologus expression of HRP especially in prokaryotic host is lack of glycosylation that affects it's stability toward...
Topics
- Amino Acid Substitution
- Asparagine
- Aspartic Acid
- Cytosol
- Escherichia coli
- Glycosylation
- Horseradish Peroxidase
- Hydrogen-Ion Concentration
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Spectrometry, Fluorescence
- Structure-Activity Relationship