Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure

1 Jan 2008

Abstract excerpt

BACKGROUND: For the accurate determination of gene expression changes during growth and differentiation studies on adipose-derived stem cells (ASCs), quantitative real-time RT-PCR has become a method of choice. The technology is very sensitive, however, without a proper selection of reference genes, to which the genes of interest are normalized, erroneous results may be obtained. RESULTS: In this study, we have...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adipose Tissue and Metabolism
Share to Mesenchymal stem cell research
Share to Molecular Biology Techniques and Applications

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 18976469
DOI: 10.1186/1471-2199-9-98

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposurePMID 18976469Read ↗

Evaluation of the stability of standard reference genes of adipose-derived mesenchymal stem cells during in vitro proliferation and differentiation.Semantic proximity Read ↗

Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineeringSemantic proximity Read ↗

TATA box binding protein and ribosomal protein 4 are suitable reference genes for normalization during quantitative polymerase chain reaction study in bovine mesenchymal stem cellsSemantic proximity Read ↗

Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.Semantic proximity Read ↗

Identification of Stable Reference Genes for Gene Expression Analysis of Three-Dimensional Cultivated Human Bone Marrow-Derived Mesenchymal Stromal Cells for Bone Tissue EngineeringSemantic proximity Read ↗

Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studiesSemantic proximity Read ↗

Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGFSemantic proximity Read ↗

Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilageSemantic proximity Read ↗

Assessment of Sex Bias in Housekeeping Gene Expression in Adipose Tissue Through the Massive Analysis of Transcriptomics DataSemantic proximity Read ↗

Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studiesSemantic proximity Read ↗

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic InjurySemantic proximity Read ↗

Evaluation of Reference Genes for Studies of Gene Expression in Human Adipose TissueSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.